Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals I. A Comparison of Isolation Procedures

نویسندگان

  • J. M. F. G. Aerts
  • A. W. Schram
چکیده

Vacuoles were isolated from the upper epiderm is o f petals o f Petunia hybrida line R27 using three different procedures. Utilizing the vacuolar localization o f anthocyanin, vacuolar prep­ arations obtained through polybase treatment, osm otic shock and m echanical forces were com ­ pared on purity, yield and stability. The comparison indicated that application o f the polybase procedure results in the best vacuolar preparations. Vacuoles could be obtained which m ain­ tained their acidic pH and retained their anthocyanin content.

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تاریخ انتشار 2013